Selected publications

Lehman KM*, May KL*, Marotta J, Grabowicz M. (2023). Genetic analysis reveals a robust and hierarchical recruitment of the LolA chaperone to the LolCDE lipoprotein transporter. mBio (accepted) PubMed link…

Marotta J, May KL, Bae CY, Grabowicz M. (2023). Molecular insights into Escherichia coli Cpx envelope stress response activation by the sensor lipoprotein NlpE. Mol Microbiol 119:586-595 PubMed link…

Smith HC, May KL, Grabowicz M (2023). Teasing apart the evolution of lipoprotein trafficking in gram-negative bacteria reveals a bifunctional LolA. Proc Natl Acad Sci U S A 120(6):e2218473120. PubMed link…

Lehman KM, Smith HC, Grabowicz M (2022). A biological signature for the inhibition of outer membrane lipoprotein biogenesis. mBio 13(3):e0075722. PubMed link…

Grabowicz, M. and Weiss D (2022). Editorial overview: Antibiotics special issue. Curr Opin Microbiol. 65:v-vii. PubMed link…

Chaudhry W., Lee, E., Worthy, A., Weiss, Z., Grabowicz M., Vega N.M., and Levin B. (2020). Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria. FEMS Microbiology Ecology 96(1):fiaa162. PubMed link…

Hart EM, Mitchell AM, Konovalova A, Grabowicz M, Sheng J, Han X, Rodriguez-Rivera FP, Schwaid AG, Malinverni JC, Balibar CJ, Bodea S, Si Q, Wang H, Homsher MF, Painter RE, Ogawa AK, Sutterlin H, Roemer T, Black TA, Rothman DM, Walker SS, Silhavy TJ. (2019). A small-molecule inhibitor of BamA impervious to efflux and the outer membrane permeability barrier. Proc Natl Acad Sci U S A 116(43):21748-21757. PubMed link…

Lehman, K.L., and Grabowicz M. (2019). Countering Gram-negative antibiotic resistance: recent progress in disrupting the outer membrane with novel therapeutics. Antibiotics 8:163. Link

May, K.L., Lehman, K.M., Mitchell, A.M., and Grabowicz M. (2019). A stress response monitoring lipoprotein trafficking to the outer membrane. mBio 10(3) pii: e00618-19. PubMed link…

Commentary highlighting our work:

• Sampson, B.W., and Trents M.S. (2019) Emerging Roles for NlpE as a Sensor for Lipoprotein Maturation and Transport to the Outer Membrane in Escherichia coli. mBio 10(3) pii: e01302-19. PubMed link…

May, K.L., and Grabowicz M. (2018). The bacterial outer membrane is an evolving antibiotic barrier. Proc Natl Acad Sci U.S.A. 115(36):8852-8854. PubMed link…

Grabowicz, M. (2018). Lipoprotein transport: greasing the machines of outer membrane biogenesis. Bioessays (doi: 10.1002/bies.201700187). PubMed link…

Konovalova A*, Grabowicz M*, Balibar CJ, Malinverni JC, Painter RE, Riley D, Mann PA, Wang H, Garlisi CG, Sherborne B, Rigel NW, Ricci DP, Black TA, Roemer T, Silhavy TJ, Walker SS (2018). Inhibitor of intramembrane protease RseP blocks the σE response causing lethal accumulation of unfolded outer membrane proteins. Proc Natl Acad Sci U.S.A. 115(28):E6614-E6621. *equal contribution PubMed link…

Lee J., Sutterlin H.A., Wzorek J.S., Mandler M.D., Hagan C.L., Grabowicz M., Tomasek D., May M.D., Hart E.M., Silhavy T.J., Kahne D. (2018). Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion. Proc Natl Acad Sci U.S.A. 115(10):2359-2364. PubMed link…

Grabowicz, M., and T.J. Silhavy (2017). Redefining the essential trafficking pathway for outer membrane lipoproteins. Proc Natl Acad Sci U.S.A. 114(18):4769-74. PubMed link…

Grabowicz, M., and T.J. Silhavy (2017). Envelope stress responses: an interconnected safety net. Trends Biochem Sci. 42(3):232-42. PubMed link…

Silhavy, T.J., Grabowicz, M., Kahne, D., Lebar, M., Andres, D. (2017). Modified lipopolysaccharide glycoform and method of use. United States Patent and Trademark Office, US Patent No. 9677052

Lee, J., Xue, M., Wzorek, J.S., Wu, T., Grabowicz, M., Gronenberg, L.S., Sutterlin, H.A., Davis, R.M., Ruiz, N., Silhavy, T.J., Kahne, D. (2015). Characterization of a stalled complex on the β-barrel assembly machine. Proc Natl Acad Sci U.S.A. 113(31):8717-22. PubMed link…

Balibar CJ, Grabowicz, M. (2015). Mutant alleles of lptD increase the permeability of Pseudomonas aeruginosa and define determinants of intrinsic resistance to antibiotics. Antimicrob Agents Chemother. 60:845-54. PubMed link…

Grabowicz, M., Koren, D., Silhavy, T.J. (2015). The CpxQ sRNA negatively regulates Skp to prevent mistargeting of β-barrel outer membrane proteins into the cytoplasmic membrane. mBio. 7(2):e00312-16. PubMed link…

Malojčić, G., Andres, D., Grabowicz, M., George, A.H., Ruiz, N., Silhavy, T.J., Kahne, D. (2014). LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface. Proc Natl Acad Sci U.S.A. 111(26):9467-72. PubMed link…

Grabowicz, M., Andres, D., Lebar, M.D., Malojčić, G., Kahne, D., Silhavy, T.J. (2014). A mutant Escherichia coli that attaches peptidoglycan to lipopolysaccharide and displays cell wall on its surface. eLife 3:e05334. PubMed link…

Grabowicz, M.*, Yeh, J.*, Silhavy, T.J. (2013). Dominant negative lptE mutation that supports a role for LptE as a plug in the LptD barrel. J Bacteriol. 195(6):1327-34. * denotes equal contribution PubMed link…